Program CANOCO Version 4.0 September 1997 - written by Cajo J.F. ter Braak Copyright (c) 1988-1997 Centre for Biometry Wageningen, CPRO-DLO Box 100, 6700 AC Wageningen, the Netherlands. CANOCO performs (partial) (detrended) (canonical) correspondence analysis, principal components analysis and redundancy analysis. CANOCO is an extension of Cornell Ecology program DECORANA (Hill,1979) For explanation of the input/output see the manual or Ter Braak, C.J.F. (1995) Ordination. Chapter 5 in: Data Analysis in Community and Landscape Ecology (Jongman, R.H.G., Ter Braak, C.J.F. and Van Tongeren, O.F.R., Eds) Cambridge University Press, Cambridge, UK, 91-173 pp. *** Type of analysis *** Model Gradient analysis indirect direct hybrid linear 1=PCA 2= RDA 3 unimodal 4= CA 5= CCA 6 ,, 7=DCA 8=DCCA 9 10=non-standard analysis Type analysis number Answer = 2 *** Data files *** Species data : OEDO_API.DTA Covariable data : TIMESITE.DTA Environmental data : TOXICANT.DTA Initialization file: Forward selection of envi. variables = 0 Scaling of ordination scores = 1 Diagnostics = 1 File : OEDO_API.DTA Title : count of Oedothorax apicatus females in control and impact site (van der Voet 19 Format : (I5,F4.0) No. of couplets of species number and abundance per line : 0 No samples omitted Number of samples 44 Number of species 1 Number of occurrences 44 File : TIMESITE.DTA Title : time and site indicators of Oedothorax apicatus data (van der Voet 1987) Format : (I5,2(I3,F3.0)) No. of covariables : 24 No interaction terms defined ****************************************************************************** Names of covariables ****************************************************************************** t1 t2 t3 t4 t5 t6 t7 t8 t9 t10 t11 t12 t13 t14 t15 t16 t17 t18 t19 t20 t21 t22 I-site C-site ****************************************************************************** File : TOXICANT.DTA Title : Spraying indicator and putative impact size (= 9 - time since spraying) Format : (I5,F2.0,F3.0) No. of environmental variables : 2 No interaction terms defined ln(Ay+B)-transformation of species data A = 1.000 B = 1.000 No species-weights specified No sample-weights specified Centring/standardization by species = 1 Centring/standardization by samples = 0 No. of active samples: 44 No. of passive samples: 0 No. of active species: 1 Total sum of squares in species data = 143.064 Total standard deviation in species data TAU = 1.80318 Covariable t22 is linearly dependent, hence ignored Covariable C-site is linearly dependent, hence ignored 1 **** Correlation matrix **** SPEC AX1 1.0000 SPEC AX2 .7978 1.0000 SPEC AX3 .0000 .0000 .0000 SPEC AX4 .0000 .0000 .0000 .0000 ENVI AX1 .6030 .0000 .0000 .0000 1.0000 ENVI AX2 .0000 .0000 .0000 .0000 .0000 .0000 ENVI AX3 .0000 .0000 .0000 .0000 .0000 .0000 .0000 ENVI AX4 .0000 .0000 .0000 .0000 .0000 .0000 .0000 .0000 Sprayed .6030 .0000 .0000 .0000 1.0000 .0000 .0000 .0000 SPEC AX1 SPEC AX2 SPEC AX3 SPEC AX4 ENVI AX1 ENVI AX2 ENVI AX3 ENVI AX4 N name (weighted) mean stand. dev. inflation factor 1 SPEC AX1 .0000 .2688 2 SPEC AX2 .0000 .2145 3 SPEC AX3 .0000 .0000 4 SPEC AX4 .0000 .0000 5 ENVI AX1 .0000 .1621 6 ENVI AX2 .0000 .0000 7 ENVI AX3 .0000 .0000 8 ENVI AX4 .0000 .0000 1 Sprayed .2045 .4034 2.6923 **** Summary **** Axes 1 2 3 4 Total variance Eigenvalues : .026 .046 .000 .000 1.000 Species-environment correlations : .603 .000 .000 .000 Cumulative percentage variance of species data : 36.4 100.0 .0 .0 of species-environment relation: 100.0 .0 .0 .0 Sum of all unconstrained eigenvalues .072 Sum of all canonical eigenvalues .026 The sum of all unconstrained eigenvalues is after fitting covariables Percentages are taken with respect to residual variances i.e. variances after fitting covariables *** MESSAGE *** The fit for species is additional to the fit due to covariables. With covariables, CANOCO cannot calculate residuals for samples. 1 *** Sample arrangement in permutation test *** Whole plot 1 : 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 Whole plot 2 : 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 These 2 whole plots are not permuted The 22 split plots are permuted completely at random using the same permutation in each whole plot Seeds: 8036 25870 **** Summary of Monte Carlo test **** Test of significance of all canonical axes : Trace = .026 F-ratio = 11.426 P-value = .015 ( 199 permutations under reduced model)